Transfection of WEHI 231 cells with NS5a-mCherry, stimulated with anti-IgM, monitored every 12 hours

Today the NS5A-mCherry transfection was repeated. Instead of photographing the cells every 24 hours for 48 hours before mounting, the cells were photographed every 12 hours for 48 hours before mounting. At 12 hours post transfection 1mL of the both the mock and transfected cells will be stimulated with 20uL of anti-IgM. At 36 hours post transfection (24 hour stimulation) the stimulated cells will be mounted. The unstimulated cells will be mounted at 48 hours.