WEHI 231 cells were transfected with NS5A-mCherry. The plasmid used came from the last round of propagation so 25 uL had to be used. It may be necessary to use 50 uL depending on outcome of this experiment. A 24 well plate was used instead of a 6 well plate. This transfection will be stimulated for 1 hour with anti-IgM after 24 hours. 750 uL of transfected cells was added to 750 uL of media in each well.