Michaela Sangillo NS5A-mCherry Transfection, ER tracker and Bodipy staining, cells prepared for flow cytometry January 8, 2015 sangillo The WEHI 231 cells were transfected with NS5A-mCherry. 12 hours after stimulation cells were stimulated with anti-IgM. At T=36 cells were collected and stained with either ER tracker or Bodipy. Cells were then prepared for flow cytometry