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Michaela Sangillo

NS5A-mCherry Transfection, ER tracker and Bodipy staining, cells prepared for flow cytometry

January 8, 2015 sangillo

The WEHI 231 cells were transfected with NS5A-mCherry. 12 hours after stimulation cells were stimulated with anti-IgM. At T=36 cells were collected and stained with either ER tracker or Bodipy. Cells were then prepared for flow cytometry

Anti-IgMB cellsCell StainingFlow CytometryNS5AStimulationTransfection

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Previous PostNS5A-mCherry transfection with anti IgM stimulation, cells prepared for PCR

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  • NS5A-mCherry Transfection, ER tracker and Bodipy staining, cells prepared for flow cytometry
  • NS5A-mCherry transfection with anti IgM stimulation, cells prepared for PCR
  • Jurkat Growth Curve
  • Preparing cells for Flow Cytometry and Electron Microscopy
  • NS5A Transfection with either Bodipy or ER Tracker staining

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Anti-IgM Autoantibodies Autoimmunity B cells Cell Staining EFR3B Electron Microscopy ER Stress Flow Cytometry Lupus Microgravity NS5A Open Access Open Notebooks Open Science PCR PI4KIIIa Plasmids Stimulation T Cells Transfection Ttc7

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