Category Archives: Molecular Biology

Activation of Stat6 in Ttc7-fsn B cells

These are some figures from my lab examining B cells from the Autoimmune prone flaky skin mutant mice.

Purified splenic B cells were unstimulated (0) or stimulated with IL-4 for 15 and 30 minutes, then stained for Stat6 (red) and the nucleus (green) and visualized with confocal microscopy using a 60x objective. Co-localization of Stat6 with the nucleus, denoting activation, is yellow. Ttc7fsn B cells show more yellow, activated cells than normal even without stimulation, suggesting pre-activation in vivo.